Bacterial Toxin Research Citations

Experimental Autoimmune Encephalomyelitis Model:

EAE was elicited and scored as previously described ³³. Briefly, on day 0 mice received bilateral subcutaneous flank injection of 50 µg of MOG (MEVGWYRSPFSRVVHLYRNGK) peptide in complete Freunds adjuvant (CFA) containing 500 µg of heat-inactivated Mycobacterium tuberculosis (Difco Labs). A dose of 200 ng of Bordetella pertussis toxin (PT; LIST Biological Labs) was injected i.p. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunohistochemical experiments:

Retrograde labeling of phrenic motor neurons. To identify phrenic motor neurons, 12 rats were used for retrograde labeling with cholera toxin B (CtB) subunit via intrapleural injection (Mantillaetal.,2009).  Isoflurane anesthesia was induced in a closed chamber and maintained via nose cone (1.5% isoflurane in 100% oxygen). A total of 25 µl of 0.5% CtB subunit (dissolved in 0.9% sterile saline; List Biological Laboratories) was injected into the right and left thoracic cavities (6 mm deep, fifth intercostal space) using a 25 µl Hamilton syringe and a custom-made needle …
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of EAE with MOG₃₅₅₅ peptide:

EAE was induced as described previously [21], [23], [24] by subcutaneous injection into the mouse flank on day 0 with 300 µg of MOG₃₅₅₅ peptide thoroughly emulsified in complete Freund’s adjuvant (CFA) containing 500 µg of heat-inactivated Mycobacterium tuberculosis (Difco, Detroit, MI). One week later (day 7), the mice were boosted with 300 µg of MOG₃₅₅₅ peptide subcutaneously in the other flank. 500 ng Pertussis toxin (List Biologicals, Campbell, CA) in 200 µl of PBS was injected intraperitoneally on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Immature DCs with or without nicotine (NiDCs and MoDCs, respectively) were cultured with 10 ng/ml lipopolysaccharide (LPS; Salmonella minnesota; List Biological Laboratories, INC, Campbell, CA) to induce cytokine and chemokine production. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Chemical inhibition of RhoGTPases:

Difficile toxin B (TcdB, List Biologicals, Campbell, CA) was used to pharmacologically inhibit Rho, Rac and Cdc42 …

• Product #155 – Toxin B from Clostridium difficile

ELISA:

All ELISAs were read at 450 nm using a 1420 Victor3 (PerkinElmer) plate reader, unless otherwise indicated. Total Ig in cell culture supernatant was quantied by IgM, IgG, or IgA ELISA kits (Zeptometrix), used according to manufacturers instructions. To monitor Ag-specic Ig production, 96-well Maxisorp plates (Nunc) were coated with either heat-killed Opa2 gonococci (N302), keyhole limpet hemocyanin (KLH; Sigma-Aldrich), or tetanus toxoid (TT; List Biological Labs) resuspended in pH 7.4 PBS (Wisent). …

• Product #191 – Tetanus Toxoid from Clostridium tetani

Animal experimentation:

… For immunization, mice received 500 mg H. pylori SS1 whole-cell sonicate along with 10 mg cholera toxin (CT; List Biologicals, Campbell, CA) by oral gavage in a total volume of 200 ml three times at weekly intervals. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Mice and Treg depletion:

… To deplete Treg cells, DEREG mice were injected with DT (List Biological Laboratories). Mice were injected i.p. with 1 mg DT every other day over a period of 9 d starting with the rst injection 1 d prior MHVA59 infection. For adoptive transfer experiments, recipients were treated with DT on days 5, 7, and 9 postinfection (p.i.). Control mice (B6) received the same DT treatment as DEREG mice. 2D2 3 DEREG donor mice were injected with 1 mg DT 1 d prior to cell isolation.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… and the retrograde tracer CTb (low salt, 1% in distilled water; List Biological Laboratories, Campell …  #104, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Immunization was performed by mixing MOG peptide in complete Freund adjuvant containing 5 mg/mL heat-killed H37Ra, strain of Mycobacterium tuberculosis (Difco Laboratories, Detroit, MI). Pertussis toxin (200 ng, List Biological Laboratories, Campbell, CA) in PBS was administered iv on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)