Bacterial Toxin Research Citations

… We attempted to induce breach of the BBB by injection of complete Freund’s adjuvant (CFA, Sigma, Gibco Laboratories and Difco, containing heat-killed H37Ra Mycobacterium tuberculosis [MTB]) and pertussis toxin (PTx, List Biological Laboratories Inc) before passive transfer …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Adoptive transfer of 2D2 T cells and induction of EAE:

… For induction of EAE in RAG-1^/ mice, mice of 8-12 wks of age first received purified 2D2 T cells (1 10⁶/mouse) i.v. Twenty four hours later, 2D2 T-cell recipients were immunized s.c. with 200 jig MOG 35-55 in CFA (containing 400 jig of Mycobacterium tuberculosis) in a total volume of 100 mL. Mice received 100 ng of pertussis toxin (List Biological, Campbell, California, USA) in 200 L PBS in the tail vein immediately after the immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell culture:

N18TG2 neuroblastoma cells (passage numbers 2550) were maintained at 37°C under a 5% CO₂ atmosphere in complete media comprising: Dulbecco’s modified Eagle’s medium … Where indicated, N18TG2 cells were pretreated with receptor antagonists or other inhibitors prior to addition of CB₁ receptor agonists. Pertussis toxin (100 ngmL¹; List Biological Laboratories, Campbell, CA, USA) was added to cells 16-20 h before addition of agonists.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE:

MOG peptide 3555 (MEVGWYRSPFSRVVHLYRNGK), purchased from Genemed Synthesis (San Antonio, TX), was used as the immunogen. Mice of 8-12 wk of age were immunized s.c. with 200 mg MOG peptide in CFA (containing 400 mg Mycobacterium tuberculosis) in a total volume of 100 ml. Mice also received 150 ng pertussis toxin (List Biological, Campbell, CA) in 200 ml PBS via the tail vein immediately after the immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE model:

Age- (7-12 wk of age) and sex-matched mice were immunized s.c. with 100 mg myelin oligodendrocyte glycoprotein (MOG)3555 peptide (MEVGWYRSPFSRVVHLYRNGK) emulsied in CFA (Difco Laboratories) with 500 mg Mycobacterium tuberculosis on day 0. Mice also received 200 ng pertussis toxin (List Biological Laboratories) by i.p. injection on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Inhibitor treatment:

For some experiments, cells were pretreated for 1 h at 37°C with a Gai inhibitor (1 mg/ml pertussis toxin; List Biologicals, Campbell, CA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To test this hypothesis, we used immunofluorescence to determine if Y1R are present in PVN neurons that project to RVLM, identified by prior RVLM microinjection of the retrograde tracer cholera toxin subunit B (CTb, 0.25%; List Laboratories). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… For the injection of cholera toxin subunit B (CtB) into LHb, a glass micropipette with an outside tip diameter… was pulled on a Flaming-Brown micropipette puller (Sutter Instrument Co, CA, USA) and filled with 1% CtB (List Biological Laboratories, Campbell, CA, USA …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Iontophoretic injections of 0.5% CTb (List Biologicals, Campbell, CA, USA) dissolved in 0.01 M phosphate buffer (PB; pH 7.4) were done by applying a 0.5- to 1.0-A positive current (200 ms pulses at 2 Hz for 15 min) through a chlorinated silver wire placed in a glass pipette …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Drugs and treatments:

… For pertussis toxin (PTx) experiments, cells were pretreated with PTx (List Biological Laboratories, Inc.) at a final concentration of 200 ng/ml or vehicle (in serum-free DMEM) for 24 hours, and PTx was maintained during drug stimulation. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)