Bacterial Toxin Research Citations

Anatomical Analysis:

… For visualization of CTB-labeled sensory axons, endogenous peroxidase activity was blocked with 0.6% hydrogen peroxide and non-specific antibody reactions were blocked with 5% horse serum for 1 hr at room temperature. Sections were incubated for 72 hours at 4C with the primary CTB antibody (goat polyclonal 1:80,000 dilution, List Biological Labs) followed by incubation …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Stimulation experiments:

… Cells were stimulated with the following agents: … 100 ng/ml lipopolysaccharide (LPS) from Esherichia coli (List Biological Laboratories), …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… The induction protocol included administration of 500 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) intraperitoneally on days 0 and 2 and emulsions of 400 mg of MOG 35-55 peptide (myelin oligodendrocyte glycoprotein, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Emulsions were prepared by mechanical Omni Mixer homogenizer and injected subcutaneously at four sites (i.e. in each flank). Pertussis toxin (300 ng/mouse; List Biological Labs, Inc., Campbell, CA, USA) in PBS was injected intraperitoneally at the time of immunization and at 72 h. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

… PTX was from List Biological Laboratories (Campbell, CA). …

Results:

… LDL activates ERK1/2 and JNK through a PTX-sensitive pathway. Quiescent RMC were serum deprived overnight in the presence or absence of 500 ng/ml PTX before stimulation for 5 min with 50 µg/ml LDL or 5 µm S1P. Basal (NS), LDL-, and S1P-stimulated phosphorylation of ERK1/2 and JNK in whole-cell lysate samples was determined as described. Representative phospho-ERK1/2 (A) and phospho-JNK (B) immunoblots are shown above bar graphs depicting mean sd for four independent experiments. The change in phosphorylation ERK1/2 and JNK are expressed as the percent above the basal level in unstimulated cells not exposed to PTX. *, P < 0.001 vs. unstimulated (NS); and #, P < 0.001 stimulated vs. PTX treated. NS, Not stimulated. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Each mouse received 200 ng pertussis toxin (PTx; List Biological Laboratories, Hornby, ONT, Canada) in 500 µL sterile phosphate-buffered saline (PBS) on the day of immunization and 48 h later. Clinical symptoms …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

… PTX was purchased from List Biological Laboratories (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PA63 and LF were purchased from List Biological Laboratories (Campbell, CA). …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

ELISA:

… An in-house ELISA was used for measuring anti-diphtheria toxoid (DT). In brief, plates (Medisorp, Thermo Scientific Nunc) were coated with 50l/well DT (1g/ml in PBS) (List Biological Laboratories, Campbell, CA, USA) and blocked with 3% BSA in T-PBS. The sera samples were diluted 1:100 in 3% BSA in T-PBS. Anti-human IgG antibody conjugated with HRP and a substrate was used in the manner described above.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In vivo depletion experiments:

To deplete CD207+ DCs or LCs, 1 g of DT (List Biological Laboratories) was injected i.p. in Lang-DTR mice at day 3 or 15, respectively. DEREG mice were depleted of CD4+Foxp3+ Tregs (8) by i.p. injection of 1 g DT at days 2 or 1 with respect to day 0 of LC transfer.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae