Bacterial Toxin Research Citations

Reversible inactivation of FLS2 cortex:

… Initially, the FLS1 cortex was micro-injected with 0.2 µl of the retrograde tracer cholera toxin subunit (1% CTB dissolved in 0.1 M PBS; List Biological Laboratories). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell culture:

Cells were cultured at 37C and in 5% CO₂ in a humidified incubator. Primary mouse BMDMs were prepared as previously described (Gilchrist et al., 2006). In brief, femoral BM cells were plated on nontissue culturetreated plastic in BMDM medium … On day 7, cells were stimulated as indicated in the Results section, before being used for microscopy, flow cytometry, RNA extraction, or lipid extraction. Stimuli that were introduced into BMDM medium (as indicated in the text) were as follows: LPS (from Salmonella minnesota; List Biological Laboratories), …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Boosting immunization of mice.

On day 117, we randomly assigned primed mice in one of several cohorts to receive a boosting vaccination (Table 1). Some cohorts received CHO-BSA (10 g saccharide/immunization/mouse) transcutaneously (TCI) with or without 25 g of immunoadjuvantative cholera toxin (CT; List Biological Laboratories, Campbell, CA) or E. coli heat-labile toxin (LT) (9). We immunized additional cohorts with CHO-BSA (10 g saccharide/immunization/mouse) subcutaneously (SCI) with or without 5 g of immunoadjuvantative CT. … Control mice received CT or LT boosting alone or no boosting immunizations following oral priming. Additional control cohorts received no priming oral immunization and received only CHO-BSA with CT or LT transcutaneously or subcutaneously. When booster doses were given, they were administered at 2-week intervals for a total of four immunizations. Transcutaneous immunizations were carried out as previously described (32).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… were transferred into complete keratinocyte serum-free medium (cKSFM) with bovine pituitary extract (50 µg/ml; Gibco) and human recombinant epithelial growth factor (5 ng/ml), and supplemented with cholera toxin (CT, 30 ng/ml; List Biological Laboratories, Campbell, CA …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Brainstem histology:

Mice were initially anesthetized with isoflurane and then given an intraperitoneal urethane injection … The sections were incubated overnight with primary antibodies against CT- (polyclonal goat antiserum to purified CT-, choleragenoid isolated from Vibrio cholerae type Inaba 569B) (product no. 703, lot no. 7032A5, diluted 1:10,000; List Biological Laboratories, Campbell, CA). … Western blot (manufacturer’s technical information). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Stimulation of human cells by Mtb:

Human THP-1 and U137 cell lines and ex-vivo peripheral blood mononuclear cellss were plated in RPMI 1640, HeLa cells were cultured in DMEM and A549 cells were cultured in F12K, all containing 10% FCS, 100Uml¹ penicillin, 100gml¹ streptomycin, and 2mM L-glutamine. 5 10⁵ cells were plated in 24-well plates overnight and stimulated with either Mtb, LPS, PAM2 or PAM3 (Mtb is whole-cell lysate of Mtb H37Rv (Colorado State University); LPS from Salmonella Minnesota (List Biological Laboratories, Campbell, CA, USA); …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Analysis of parasite replication in macrophages:

Mutants were rst screened in 96-well plates containing bone marrowderived macrophages. Equal volumes of parasites were transferred from 96-well plates containing HFF cells to 96-well plates containing macrophages. Parasites were allowed to infect macrophages for 4 h and then plates were rinsed with D10 media to remove extracellular parasites. D10 media supplemented with 100 ng/ml LPS (Escherichia coli; List Biologicals, Campbell, CA), and …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Experimental Autoimmune  Encephalomyelitis (EAE) Induction:
 
MOG-dependent EAE was induced in 6- to 8-week old female C57BL/6 mice via
a single peptide dose.  MOG35-55 (Bio-Synthesis, Inc. Lewisville, TX) peptide was
emulsified in complete Freunds adjuvant (CFA) with 4 mg/mL of killed Mycobacterium
tuberculosis, which was subcutaneously (s.c.) injected in 100 µL into the flank of
anesthetized mice on Day 0.  Mice also received 200 ng of pertussis toxin (PT; List
Biological Laboratories, Inc., Campbell, CA) intraperitoneally (i.p.) on Day 0 and Day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mice were also injected i.p. with 400 ng Bordetella pertussis toxin (List Biological Laboratories) at the time of and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

SJL Mice Immunization:

… At day 7, immunization boost was performed by delivering another identical subcutaneous injection of emulsion on the second shoulder. Mice were also injected intraperitoneally with 100 mL of a pertussis toxin solution (500 ng/100 mL; List Biological Laboratories, Campbell, CA) at days 0, 1, 7, and 8.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)