Bacterial Toxin Research Citations

… All animals were kept on a 12h dark-light cycle and standard housing conditions with ad libitum food access. At postnatal day 5 (P5), C57Bl/6 mice were randomly assigned to 4 experimental groups: lipopolysaccharides (LPS) (1 mg/kg, #423, List Biological Laboratories, Campbell, CA, USA)-injected mice …

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

… For immunolabeling, sections were blocked with 10% horse serum (Thermo Fisher Scientific, Waltham, MA, USA) in 0.1 M TBS, followed by the overnight incubation with primary antibody against choline acetyltransferase (ChAT, goat, 1:200, AB144P; Sigma-Aldrich, St. Louis, MO, USA) or CTB (goat, 1:500, 703; List Biological Laboratories, Inc., Campbell, NJ, USA) at 4 °C. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

2.4. EAE Induction and Scoring

Eight-week-old mice were immunized subcutaneously on the back with two injections of 150 μg myelin oligodendrocyte glycoprotein peptide (MOG35–55, GenScript, Piscataway, NJ, USA) emulsified in complete Freund’s adjuvant (CFA; Difco Laboratories, Franklin Lakes, NJ, USA) containing 2.5 mg/mL heat-killed Mycobacterium tuberculosis. Mice were further injected intraperitoneally with 200 ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.1 mL of PBS at 0 and 48 h postimmunization with MOG35–55. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE

EAE was induced with 50 μg of myelin oligodendrocyte glycoprotein fragment MEVGWYRSPFSRVVHLYRNGK (MOG35–55 peptide; #crb1000205n Cambridge Research Biochemicals) mixed with incomplete Freund’s Adjuvant (#263910, BD) containing 4 mg/ml Mycobacterium tuberculosis TB H37 Ra (#231141, BD), at a ratio of 1:1 (v/v). Mice received two subcutaneous injections of 100 μl each of the MOG/CFA mix. Mice received a single intraperitoneal injection of pertussis toxin (#180, List Biological Laboratories) at a concentration of 1 ng/μL in 200 μL of PBS. A second injection of pertussis toxin at the same concentration was inoculated two days after the EAE induction. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… (CFA) at a dose of 100 µg per mouse, followed by the administration of pertussis toxin (PT) (200 ng per mouse through intraperitoneal injection (IP); List biological …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Then, 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) was intraperitoneally injected on days 0 and 2 after immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… pertussis toxin (List Biological Laboratories, Campbell, CA);…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Diphtheria Toxin List Biological Laboratories 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… After immunization, 0.25ug pertussis toxin (PTX) (List Biological Laboratories, Campbell, California, USA) dissolved in PBS was injected intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Toxin degradation assay

Toxin degradation was assessed using ADS024 CFS and reconstituted lyophilized cells. The CFS was prepared as previously described and was serially diluted 1:2 in BHI broth 10 times. Lyophilized ADS024 was reconstituted and serially diluted in phosphate-buffered saline. Purified C. difficile toxins A and B (0.2 mg/mL) were obtained from List Biological Laboratories (Campbell, CA, USA). Purified toxin was reconstituted in sterile water to contain 50 mM Tris, 50 mM NaCl, pH 7.5, and 0.1% trehalose and was stored at 2–8 °C; 2.5-μL (0.2 mg/mL) aliquots of purified C. difficile toxin A or B were co-incubated for 2 h at 37 °C with 30 μL of either ADS024 CFS, or lyophilized ADS024, and the reactions were stopped, heated, and electrophoresed in a 3–8% NuPAGE Tris–acetate protein gel as 15-μL volumes/well. The gel was electrophoresed at 150 V for 50 min. Western blot using TcdA or TcdB primary antibody (1:5000 dilution from List Biological Laboratories) and fluorescent secondary antibodies was performed.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile