Citations

EAU Induction and EAU Clinical Score

The mice were subcutaneously injected with a mixture of 2 mg/mL of human interphotoreceptor retinoid-binding protein 1-20 (IRBP1-20, GPTHLFQPSLVLDMAKVLLD; GiL Biochem, Shanghai, China) and complete Freund’s adjuvant consisting of 2500 ng of mycobacterium tuberculosis (BD Difco, San Jose, CA, USA) in a 1:1 volume ratio. Additionally, 0.25 µg pertussis toxin (PTX; List Biological Laboratories, Campbell, CA, USA) was injected on the same day and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… At day 0 and day 2 after immunization, we injected 400ng of pertussis toxin (List Biologicals) intraperitoneally …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Anatomical Studies

… Sections containing the PH and the rRPa were preincubated in an antibody dilution solution (ADS: 500 mL of PBS, 0.3% Triton X-100, 1.25 g of carrageenan, 100 mg of NaN3, 5 mL of normal donkey serum) for 2 h and incubated overnight at room temperature with the primary antibodies for TH (1:1,000, rabbit-anti-TH, Millipore, AB152, RRID AB_390204), CTb (1:10,000, goat-anti-CTb, List Biological Laboratories, No. 703), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

2.3. Reagents

… Anthrax protective antigen, PA63, was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

… Pertussis toxin was obtained from List Biological Laboratories, Campbell, CA. …

MOG-Induced EAE in C57BL/6 Mice.

… The emulsification was made from equal parts of oil and liquid portions (1:1) in two syringes connected to each other with Leur lock, transferred to an insulin syringe, and 0.2 mL was injected to the right flank of each mouse. On the day of immunization, pertussis toxin (200 ng/mL) was injected i.p. at a volume dose of 0.2 mL/mouse. The injection of the pertussis toxin (i.p) was repeated after 48 h to boost the immune ecognition reaction. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Diluted blood was either left unstimulated (negative control) or stimulated with 5 µg/mL PT antigen (heat-inactivated PT, Bordetella pertussis strain 165, List Biological) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2 Regional Iba-1 responses in an in vivo ferret model of inflammation-sensitized perinatal brain injury

On the morning of P17, ferret kits were removed from the nest, weighed, and randomized to either the nonsurgical control or the hypoxic-ischemic-hyperoxic (HIH)-exposed group, as previously described.4 Briefly, controls and HIH-exposed ferrets received 3 μl/g saline vehicle or 3 mg/kg lipopolysaccharide (LPS, ultrapure from E. coli 055:B5, List Biological, CA) intraperitoneally, respectively. Animals that received LPS underwent a bilateral carotid ligation under isoflurane anesthesia (3%–5%) plus buprenorphine (0.05 mg/kg) analgesia. …

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

CHO Cell Cluster Response—Chinese Hamster Ovary (CHO) K-1 cells were obtained from ATCC and maintained in F12 + 10% FBS. For CHO cell-PT assays, CHO cells were plated in a 96-well tissue culture plate at 2.5 × 104/mL in F12 + 1% heat-inactivated (HI) FBS and grown overnight at 37 °C, 5% CO2. PT preparations purified in the Hewlett lab and obtained from List Laboratories were comparable in activity. PT was added at indicated concentrations to duplicate wells and the mixture incubated for 24 h at 37 °C, 5% CO2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction

EAE was induced in 8–10-week-old female C57Bl/6 mice (Jackson Laboratories, Bar Harbor, ME). Mice received a subcutaneous immunization at each hind flank of 50 μg of myelin oligodendrocyte glycoprotein (MOG35–55) in complete Freund’s adjuvant (CFA; Fisher Scientific, Waltham, MA) supplemented with myobacterium tuberculosis on day 0. Pertussis toxin (List Biological Laboratories, Hornby, ON, Canada) was administered via intraperitoneal injection at a dose of 300 ng/200 μL on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Administration of Drugs

DT (15046A1, List Biologicals Laboratories) was used as previously described.26 Conditional ablation of Tregs in DEREG mice was achieved by intraperitoneal injection of DT (50 µg/kg body weight) 2 days before the MCAO model. Subsequently, an intraperitoneal dose of DT (10 µg/kg body weight) was administered every other day until day 3 after MCAO. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae