Citations

… 20 ng/ml cholera toxin (List Biological Laboratories; Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… PTX was purchased from List Biological Laboratories …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Microinjection of CTB (low salt formulation; List Biological Labs, Campbell, CA, USA) was performed into the wall of selected regions of the LUT under isoflurane anesthesia (3% in 125 oxygen for induction, 1-2% for maintenance), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis Toxin (PTX) from List Biological Laboratories (Campbell, CA); …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin (CT) was purchased from List Biological Laboratories (Campbell, CA, USA). Mice were subjected to weekly intragastric (i.g.) PN sensitization from week 0 through week 5 with ground peanut (10 mg) plus CT (20 µg) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAU model induction and EAU clinical score

For active induction of EAU, mice were injected subcutaneously with an emulsion consisted of 2 mg/mL of retinal antigen interphotoreceptor retinoid-binding protein 1–20 (IRBP1–20) (GPTHLFQPSLVLDMAKVLLD, GiL Biochem, Shanghai, China) and complete Freund’s adjuvant (BD Difco, San Jose, CA, USA) containing 2.5 mg of Mycobacterium tuberculosis strain H37Ra (BD Difco, San Jose, CA, USA) in a 1:1 volume ratio. Additionally, 0.25 µg pertussis toxin (PTX) (List Biological Laboratories, Campbell, California, USA) dissolved in PBS was injected on the same day and 2 days after immunization (Chan et al., 1990; Agarwal et al., 2012; Chen and Caspi, 2019b). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2. EAE induction

EAE was induced in female and male mice at the age of 12 weeks as described earlier with some modifications (Chechneva et al., 2011). In brief, mice were injected subcutaneously at two flank sites with an emulsion of myelin oligodendrocyte glycoprotein peptide fragment that spans amino acids 35–55 (MOG) (New England Peptide) in incomplete Freund’s adjuvant supplemented with 500 μg Mycobacterium (M.) tuberculosis H37Ra (Difco). Our previously described EAE model with MOG prepared by sonication causes severe EAE in C57BL/6 mice (Chechneva et al., 2011). Sonication changes biochemical composition of MOG epitopes and/or M. tuberculosis causing an enhanced immune response (Adelmann et al., 1995). In KO mice, sonicated MOG causes mild EAE. EAE in KO and Floxed control mice was induced by MOG (300 μg) emulsion prepared by quick sonication (3 × 5 sec on ice). To induce mild EAE in WT mice, MOG (150 μg) was homogenized with 2 syringes connected by a stopcock (twice 10 min with 3 h interval on ice). Emulsion was kept in the dark at 4 °C between mixing and before preparing into individual doses in syringes. All mice received 200 ng of pertussis toxin (PTX) (List Biological Labs) intraperitoneally (i. p.) on day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Two hours later, the mice were i.p. injected with Pertussis Toxin (List Biological laboratories, Campbell, CA, USA) freshly prepared in ice-cold PBS (200ng in 100µL …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and exosomes treatment

… To induce EAE, female C57BL/6J mice were immunized with 200 μg MOG35–55 (Sinobioway Co., Ltd., Zhangzhou, China) in complete Freund’s adjuvant (Chondrex Inc., WA, USA) with 4 mg/mL heated-killed M. Tuberculosis (H37Ra, non-viable). Pertussis toxin (List Biological Laboratories Inc., CA, USA) was intraperitoneally injected at 400 ng on days 0 and 2. After 15-20 days, mice displayed the peak of the disease showing complete paralysis of the tail and hind limbs. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

4.5. Treatment of Stx2 on the Chip

Stx2 (List labs; Campbell, CA, USA) was diluted in the DMEM/F12 as supplemented with 5% (v/v) FBS, 100 unit/mL penicillin, and 100 μg/mL streptomycin. One hundred microliters of Stx2 was added only in the gut module, i.e., after assembly of the gut and kidney modules.

• Product #162L – Shiga Toxin 2 from Escherichia coli