Citations

Induction and assessment of EAE

Active EAE was induced as previously described (38). EAE induction was performed with age- and sex-matched mice. Briefly, mice were injected subcutaneously with the MOG35–55 peptide (200 μg) in Complete Freund’s Adjuvant (CFA) containing heat-killed Mycobacterium tuberculosis (5 mg/ml; H37Ra strain, BD Diagnostics). On the day of immunization and 48 hours later, mice were injected intraperitoneally with Pertussis toxin (200 ng per mouse; List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Microinjection of CTB Into the LR3 and LR8 on the Opposite Side of Hindlimb

Six rats were used for the injection of CTB into the LR3 and LR8 on the opposite sides of hindlimbs. Considering the application of
the CTB on the peripheral site only produced neural labeling on the same side of injection, bilateral injections were performed in
this study. This is also in order to reduce the number of animals used. Under the anesthesia with 2% isoflurane, 2 µl of 1% CTB
solution (List Biological Labs, Inc., Campbell, CA, United States) was injected subcutaneously and muscularly with Hamilton
micro-syringe (Hamilton Company, Reno, NV, United States) into the right side of LR3 and the left side of LR8, respectively. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

ELISPOT assay

ELISPOT was performed to enumerate cholera toxin and LPS specific plasmablast present in the PBMC samples. 96-well ELISPOT assay filter plates (Millipore) were coated overnight at 4°C with CTB (List Labs #104), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell-rounding assay.

This assay was used to measure toxin activity, as C. difficile toxins cause cell rounding of tissue cultures, and was performed as described previously (43) with slight modifications. Briefly, Vero cells (monkey kidney epithelial cells) from ATCC were aliquoted in 24-well plates at a density of 103 cells/ml and incubated overnight at 37°C in a CO2 incubator. Cells were treated with serially diluted concentrations of ebselen (ranging from 125 ng/ml to 128 μg/ml) for 30 min, followed by the addition of TcdB (List Biologicals Laboratories, Inc.) to a final concentration of 20 ng/ml. The plates were incubated at 37°C in a CO2 incubator for 2 h and then observed with phase-contrast microscope (Nikon Diaphot 200/300 inverted microscope) with counting from 5 views per well.

• Product #155 – Toxin B from Clostridium difficile

… Control animals received subcutaneous injections lacking MOG33-55 peptide. At the time of immunization and 48?h later, mice received intraperitoneal injections with 500 ng pertussis toxin (List Biologicals, cat. no. 180)

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Sections were the incubated with goat anti-CTB (List Biological Laboratories Cat #703, 1:10,000) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Mice also received 250 ng of pertussis toxin (List Biological Laboratories) ip on days 0 and 2 postimmunization (pi). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… DT (List Biological Laboratories, 150) was dissolved in PBS, and 1µg (first dose) or 250ng (subsequent doses) was injected intraperitoneally for each mouse …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Top-down lipopolysaccharide structural analysis using multistage MS (MS/MS and MS3)

To determine the structures and validate the purity of different rough-type LPS preparations, top-down LPS structural elucidation was performed as described previously (27). Highly purified LPS of Bordetella pertussis 165 strain and Escherichia coli J5 strain were obtained from List Biological Laboratories, Inc. (Campbell, CA). LPS samples were dissolved at an approximate concentration of 20 μg ml−1 in 2-propanol/water (50:50 [vol/vol]). …

• Product #301 – LPS from Escherichia coli J5 (Rc)

Rocky Hill, NJ). Bone marrowderived macrophages (BMDMs) were washed twice with D-PBS (Wisent) to remove nonadherent cells and cultured in fresh IMDM 1 10% FBS alone or containing 1000 ng/ml LPS (List Biological Laboratories, Campbell, CA) or 40 mM hemin (Sigma-Aldrich, St. Louis, MO). A