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July 27, 2022
Nature
Dibble, CC;Barritt, SA;Perry, GE;Lien, EC;Geck, RC;DuBois-Coyne, SE;Bartee, D;Zengeya, TT;Cohen, EB;Yuan, M;Hopkins, BD;Meier, JL;Clohessy, JG;Asara, JM;Cantley, LC;Toker, A;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
Maintenance culture conditions
All MCF10A-derived cell lines were maintained in standard MCF10A growth medium without antibiotics (DMEM/F12 medium (Wisent Bioproducts, 319-075-CL), 5% horse serum (Gemini Bio, 100508), 10 µg ml−1 insulin (Thermo Fisher Scientific/Gibco, A11382II), 0.5 mg ml−1 hydrocortisone (Sigma-Aldrich, H4001), 20 ng ml−1 EGF (R&D Systems, 236-EG-01M) and 100 ng ml−1 cholera toxin (List Biological Laboratories, 100B)). …
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
July 26, 2022
Scientific Reports
Moezzi, D;Dong, Y;Jain, RW;Lozinski, BM;Ghorbani, S;D'Mello, C;Wee Yong, V;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
EAE and EAE tissue isolation
Female C57BL/6 mice (8–10 weeks old) from Jackson Laboratories were immunized subcutaneously with MOG 35–55 peptide (50 μg/100 μl, Protein and Nucleic acid facility, Stanford University School of Medicine, Stanford, CA) emulsified in complete Freund’s adjuvant (CFA) (Thermo Fisher Scientific) containing 10 mg/ml of heat inactivated Mycobacterium tuberculosis H37RA (Sigma-Aldrich). 50 μl emulsion was injected at one site into each hind flank. On the day of immunization and 48 h later, animals received intraperitoneal injections of 300 ng of pertussis toxin (List Biological Laboratories). …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
July 25, 2022
ACS Infectious Diseases
Patel, EN;Lin, L;Sneller, MM;Eubanks, LM;Tepp, WH;Pellett, S;Janda, KD;
Product: SNAPtide® Peptide Substrate flP6(DABCYL/5-IAF) for C. botulinum Type A Neurotoxin
… The assay mixtures were allowed to incubate at rt for 30 min, before the addition of 25 µL of SNAPtide substrate flP6 (List Laboratories) to initiate the enzyme reaction, …
• Product #523 – SNAPtide® Peptide Substrate flP6(DABCYL/5-IAF) for C. botulinum Type A Neurotoxin
July 23, 2022
SSRN Electronic Journal
Li, M;Wang, Y;Zhang, S;Song, D;Tian, R;Wang, L;
Product: Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence
2.1 Materials and Chemicals
… The aLF was ordered from List Biological Laboratories, Inc (Listlabs, US). …
2.2 The Preparation of aLF Proteolytic Reaction
aLF, ZnCl2 and peptide substrate were mixed together as proteolytic buffer (5.64 μM, 28 mM and 2.82 μM) for endopeptidase activity reaction, …
Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence
July 23, 2022
Nature Communications
Evans, JC;McEneany, VL;Coyne, MJ;Caldwell, EP;Sheahan, ML;Von, SS;Coyne, EM;Tweten, RK;Comstock, LE;
Product: LPS from Salmonella typhimurium
Purified LPS and membrane receptor blots
LPS was purified by the hot phenol method previously described41. Purified LPS from E. coli 0111:B4 and Salmonella typhimurium were purchased from List Labs. …
• Product #201 – LPS from Escherichia coli O111:B4
• Product #225 – LPS from Salmonella typhimurium
July 22, 2022
Immunohorizons
Dittel, LJ;Dittel, BN;Brod, SA;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Induction of active EAE
SJL/J 68-wk-old females (The Jackson Laboratory, Bar Harbor, ME) were actively immunized with PLP 139151, but otherwise
maintained, handled, and surveilled as outlined previously (25). Briefly, mice were actively immunized by s.c. injection of 0.2 ml inoculum containing 1030 mg PLP 139151 (HSLGKWLGHPDKF) peptide in IFA (Difco Laboratories, Detroit, MI) with 800 mg
Mycobacterium tuberculosis hominis H37Ra on days 0 and 7 (26). These inoculations were followed with 200 ng pertussis toxin i.p. (List Biological Laboratories) on days 0 and 2. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
July 18, 2022
Cell Reports Methods
van Niekerk, EA;Kawaguchi, R;Marques de Freria, C;Groeniger, K;Marchetto, MC;Dupraz, S;Bradke, F;Geschwind, DH;Gage, FH;Tuszynski, MH;
Product: Anti-Cholera Toxin B Subunit (Goat)
Immunolabeling
The following primary antibodies were used: … CTB 1:5000 (List Biologics 703 lot# 7032A10) …
July 18, 2022
Journal Of Clinical Immunology
Yang, L;Liu, P;Du, H;Chen, R;Zhou, B;Li, Y;Zhou, L;Wang, X;Liu, C;Ding, Y;Tang, X;Chen, Y;An, Y;Zhao, X;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… ELISpot plates (Millipore) were coated with PT (0.1 µg/µl, List Biological Technology) and anti-human IgG (15 µg/ml, Biolegend) overnight at 4 °C. Coated plates were washed and blocked with RPMI medium 1640 basic …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
July 15, 2022
Iscience
Wu, Y;Mahtal, N;Paillares, E;Swistak, L;Sagadiev, S;Acharya, M;Demeret, C;Werf, SV;Guivel-Benhassine, F;Schwartz, O;Petracchini, S;Mettouchi, A;Caramelle, L;Couvineau, P;Thai, R;Barbe, P;Keck, M;Brodin, P;Machelart, A;Sencio, V;Trottein, F;Sachse, M;Chicanne, G;Payrastre, B;Ville, F;Kreis, V;Popoff, MR;Johannes, L;Cintrat, JC;Barbier, J;Gillet, D;Lemichez, E;
Product: Shiga Toxin 1 from Escherichia coli
Cell culture and bacterial toxins
… Stx1 (#161) and Stx2 (#162) were from List Biological. …
• Product #161 – Shiga Toxin 1 from Escherichia coli
• Product #162L – Shiga Toxin 2 from Escherichia coli
July 13, 2022
Biology
Rasquinha, M;Lasrado, N;Petro-Turnquist, E;Weaver, E;Venkataraman, T;Anderson, D;Laserson, U;Larman, H;Reddy, J;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
2.4. Immunization Procedures
A/J mice were subcutaneously immunized on days 0 and 7 in the inguinal and sternum regions, with Myhc-α 334–352 or SERCA2a 971–990 emulsified in complete Freund’s adjuvant (CFA) containing Mycobacterium tuberculosis H37RA extract (5 mg/mL; Difco Laboratories, Detroit, MI, USA). Pertussis toxin was administered on days 0 and 2 (100 ng/mouse, i.p) (List Biological Laboratories, Campbell, CA, USA). Sera were collected on day 0 and day 21 post-immunization at termination.
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
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