Citations

… 250 ng of pertussis toxin (List Biological Laboratories) was administered ip on the day of immunization and again after 48 h. The disease symptoms in mice were observed and scored daily according to the following scoring criteria …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and activity

Eight to twelve weeks-old mice were injected subcutaneously with 100mL of an emulsion containing 200mgofMycobacterium tuber-culosisH37Ra (Cat# DF3114-33-8, Difco) and 200mg of MOG35–55(Cat# MOG3555-P2, Genemed Synthesis) distributed over threesites on the flank. On day 0 and 2 after immunization, 200 ng pertussis toxin (Cat# 180, List Biological Laboratories) were adminis-tered intraperitoneally (i.p.). …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

B. anthracis lethal toxin (LeTx) (i.e., PA plus B. anthracis lethal factor [LF] [List Biological]) was then added to the plates. After overnight incubation, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; Sigma-Aldrich] was added to assess the viability of macrophages as a function of redox potential …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Allergic sensitization and mouse model of asthma

For allergic sensitization, mice were lightly anesthetized with isoflurane and given two oropharyngeal (o.p.) instillations, 1 week apart, of 100 μg LPS-free OVA (Worthington Biomedical) with 10 μL HDE or 100 ng LPS (Millipore Sigma) in a total volume of 50 μL in PBS (HDE/OVA or LPS/OVA)30. The HDE was prepared as previously described32,59. Briefly, vacuumed dust samples from homes in North Carolina were passed through a coarse sieve, then extracted at 100 mg/mL with PBS at 4 °C with overnight mild agitation. The samples were centrifuged to remove insoluble debris, and supernatants were sterilized by passage through a 0.22 μm filter (Millipore Sigma). Endotoxin concentration was 50 ng LPS/10 μL HDE, as measured by a Limulus Amebocyte Lysate assay (Lonza, catalog #50-648U). In some experiments, 100 μg Alexa Fluor (AF) 647-conjugated OVA or 10 μg DQ-OVA (ThermoFisher Scientific) was used to analyze antigen uptake60. In some experiments, DTX (20 ng/g body weight) (List Biological Laboratories) was injected into peritoneal cavity of zDC-DTA mice prior to airway sensitization …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

CR cultures

Both murine and human EpCAM+ cells were propagated using a Conditional Reprogramming (CR) culture protocol. In brief, EpCAM+ cells were plated on irradiated (30 gray) 3T3 cells in F-medium composed of 1:3 v/v DMEM: F-12 nutrient HAM supplemented with 5% FBS, 10 ng/ml EGF (BD Biosciences; 354052), 5 μg/ml insulin (Sigma; I2643), 24 μg/ml adenine (Sigma; A2786), 0.4 μg/ml hydrocortisone (Sigma; H4001), 10 ng/ml cholera toxin (List Biological laboratory; 100B), and 10 μM ROCK inhibitor (Y-27632; ENZO). All CR cultures were maintained at 37°C and 5% CO2. When CR cultures reached 80% confluence, they were differentially trypsinized using a two-step procedure the first to remove loosely attached feeder cells, and the second to trypsinize epithelial cells.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Cells were challenged with 0.1-1000 ng/ml LPS (Escherichia coli 055:B5; List Biological Laboratories, Campbell, CA, USA). When cells were treated with NAC (Nacalai Tesque, Kyoto, Japan), it was added 30 min before LPS stimulation …

• Product #203A – LPS from Escherichia coli O55:B5

… chime- ric mice (noted below) sc with 100 mg MOG protein emulsified in CFA (Sigma-Aldrich) containing 4 mg/ml heat-killed Mycobacterium tuberculosis (H37RA) (Difco) accompanied by two ip injections of 200 ng Bordetella pertussis toxin (List Biological Laboratories …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The distance between each nucleus and its most adjacent nucleus was decreased by purified PTx at concentrations of 3-725 mIU/mL (BRP1, CHO units) (BRP1) and 0.005-4 ng/mL (LIST Biological), as well as in the context of two aP containing vaccines …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction, treatments, and clinical evaluation of MOG-induced EAE model

For induction of EAE, female C57BL/6 mice (aged 8–10 weeks) were immunized subcutaneously (s.c.) at four spots on the flanks with 200 µl of an emulsion of MOG-peptide 35–55 (pMOG, 250 µg per mouse) in CFA (Difco, Heidelberg, Germany) supplemented with 800 µg of Mycobacterium tuberculosis H37Ra on day 0. Mice also received intraperitoneally (i.p.) 200–400 ng of Bordetella pertussis toxin (List Biological Laboratories, Campbell, USA) on days 0 and 2. Paralyzed mice were given easier access to food and water. All experiments with long observation times were not pooled for statistics, as in this model a significant variability of the time course and overall disease severity has typically to be observed. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Coupling of DT (List Biologicals, cat no #151), PT (List Biologicals, cat no #181) …

• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)