Bacterial Toxin Research Citations

… intrapleural injections of the retrograde tracer cholera toxin B fragment at a concentration of 0.2% (CTB, List Biologicals, Campbell, CA, USA) to identify phrenic motor neurons in the cervical spinal cord as previously described (Mantilla et al., 2009, Michel-Flutot et al., …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of EAU in Mouse Model

After anesthesia, each C57BL/6J mouse was subcutaneously injected with 200 µL of a mixture containing 2-mg/mL retinal antigen interphotoreceptor retinoid-binding protein 1–20 (IRBP1-20; GL Biochem, Shanghai, China) and 2.5-mg/mL Mycobacterium tuberculosis dissolved in complete Freund’s adjuvant (BD Biosciences, Franklin Lakes, NJ, USA). Additionally, 0.25 µg of pertussis toxin (PTX; List Biological Laboratories, Campbell, CA, USA) was injected intraperitoneally on the day of immunization and again 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.7 Immunohistochemistry

… A series of 30 μm-thick transverse sections containing the CeA or the preBötC was prepared with a cryostat (CM1860, Leica Biosystems, Tokyo, Japan). Sections containing the CeA were stored in 0.1 M PBS containing 0.1% sodium azide (Nacalai Tesque, Kyoto, Japan) until immunostaining. We amplified CTb-Alexa-488 via immunostaining (Arima et al., 2019). Sections were washed in 0.1 M PBS, incubated in blocking buffer (PBS containing 0.3% Triton X-100 and 3% normal donkey serum) for 1 h and then incubated overnight with a goat CTb antibody (1:10000, #703, List Labs, Campbell, CA, USA) and a rabbit monoclonal protein kinase C-delta (PKCδ) antibody (1:1000, ab182126, Abcam, Waltham, MA, USA) in blocking buffer. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

2.3. Intranasal Immunization and RSV Challenge

In experiments for immunization assay, BALB/c mice were divided into four groups: (1) control (Ctrl.), (2) RSV-F protein only (RSV-F), (3) RSV-PST, and (4) RSV-F protein with cholera toxin (RSV-CT). The mice were anesthetized with 2.5–3% isoflurane for 4 min using Apparatus Table-Top Anesthesia System for Small Rodents (Harvard Apparatus, Holliston, MA, USA) and immunized intranasally with 10 µg of RSV-F protein alone or RSV-PST including 10 µg of RSV-F for twice on days 0 and 14. As a positive control, 10 µg of RSV-F protein mixed with 1 µg of CT (list labs, Campbell, CA, USA) was immunized. The volume for intranasal immunization in each mouse was 30 µL in PBS. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… In addition, 100 ng of pertussis toxin (dissolved in 100 µL PBS; List Biological Lab, USA) was administered intraperitoneally on day 0, followed by a second dose 48 h later (Segal et al., 2020). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… immunization of mice, the prepared emulsion was mixed with equal volumes of interphotoreceptor retinoid-binding protein (1-20) (IRBP1-20; 2 mg/mL; GPTHLFQPSLVLDMAKVLLD, GiL Biochem, Shanghai, China) phosphate buffer saline (PBS) solution. Injections of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) were administered intraperitoneally on day 0 and day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell lines and cultures

… Briefly, early-passage (<6) OC49 cells were plated on mitomycin C pretreated 3T3-J2 fibroblasts grown in iron-supplemented calf serum (HyClone, SH30072), expanded to a 10-cm dish, replated on tissue culture–treated dishes, and used for experimental analyses at 48 hours. PDX Standard Media consists of 60% Ham’s F-12 media (Gibco, 11765054), 35% DMEM (Gibco, 11995073), 5% FBS (R&D Systems, S12450), 0.4 μg/mL hydrocortisone (Stem Cell Technologies, 07904), 5 μg/mL insulin (Gibco, 12585014), 8.4 ng/mL cholera toxin (List Biological Laboratories Inc., 100B), 10 ng/mL EGF (Gibco, PHG0313), 24 μg/mL adenine (Alfa Aesar, A14906), 10 μmol/L ROCK inhibitor (Tocris, Y-27632), 1× penicillin–streptomycin (Gibco, 15140122), and 1× L-glutamine (Gibco, 25030081). Cells were checked every 6 months using MycoScope PCR Detection Kit (Genlantis, MY01100). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Unnicked DT from Corynebacterium diphtheriae was purchased from List Biological Laboratories. Zbtb46-DTR bone marrow chimeras were injected i.p. with a first dose of 640 ng DT per mouse, …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Immunization was carried out at days 0, 7, 14, 21 and 28. Additionally, PTX (List Labs, cat. #: 181, Campbell, California, USA) …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis toxin (PTx) was obtained from List Biological 134 Laboratories (Campbell, CA, USA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)