Bacterial Toxin Research Citations

2.1.1. Experimental autoimmune encephalomyelitis (EAE) model

In brief, male and female C57BL/6 J mice were injected subcutaneously at two sites with an emulsion of 100 μl complete Freund’s adjuvant prepared by mixing incomplete Freund’s adjuvant (BD Biosciences) and Mycobacterium tuberculosis (BD Biosciences) and 100 μg MOG 35–55 (Bio-Synthesis Inc.). Mice received 200 ng bordetella pertussis (List Biological Laboratories, Inc) intraperitoneally at day 0 and day 2 after EAE induction. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Briefly, mice received two ip injections of pertussis toxin (PTX) (List Biological Laboratory, #181) dissolved in phosphate-buffered saline (PBS) (400 ng/mouse; day 0 and …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Experimental animals

… The retrograde neuroanatomical tracer unconjugated CTB (List Biological Laboratories, Campbell, CA) was pressure-delivered into the caudate nucleus of one hemisphere and the putamen of the contralateral hemisphere in four monkeys (bilateral injections); whereas in one additional monkey, CTB was delivered into the GPi of one hemisphere and into the GPe of the contralateral hemisphere. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Diphtheria Toxin from Corynebacterium Diphtheria (unnicked) List Biological Laboratories Inc Cat# 150 …

Tissue dissection, islet isolation and cell culture

… Islets and myeloid fractions obtained from each pancreas were combined by overnight culture in AggreWells (Stem Cells Technology) in RPMI-10% FCS-5mM glucose; recovered islets/myeloid cell aggregates were then cultured up to 10 days in the same medium in the presence or absence of 50 ng/ml of DT. Culture medium and DT were replaced every 48 hours.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… FoxP3DTR mice to receive transferred Treg cells were treated with 50 mg/kg diphtheria toxin (DT) (List Labs, 150) starting from 5 days prior to tumor inoculation and for every 3 days until time of euthanization. Mice were monitored daily for tumor growth. Tumor volume was measured through digital calipers along the x, y, and z axes and calculated as (xyz)/2. Mice were to be euthanized if tumors exceeded size limit agreed by the Institutional Review Board (IRB), however no animal reached this volume during the duration of the experiment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

EAE immunization
8-12 weeks-old male and female mice, minimum of 3 per gender and genotype, were subcutaneously injected at three sites with 200 µg of MOG peptide 35–55 emulsified in Complete Freund’s Adjuvant (CFA) containing 400 µg of Mycobacterium (Cat#: EK-0111, Hooke Laboratories). On day 0 (D0) and 1 day after (D1) immunization, mice were intraperitoneally injected with 200 ng of pertussis toxin (Cat#: 180, List Biological Laboratories). …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Tetanus toxoid fluorophore conjugation Tetanus toxoid (191B; List Labs) was conjugated using the Lightning Link FITC conjugation kit (ab102884; Abcam) per manufacture’s instruction. …

• Product #191 – Tetanus Toxoid from Clostridium tetani

… Male 6-8 weeks old C57BL/6J/Sja mice (Hunan Slack King Experimental Animal Company) were maintained under specific pathogen-free conditions. Ultra Pure LPS (Escherichia coli 0111:B4, LIST Biological Laboratories, Campbell, USA) was dissolved in sterile normal saline and injected intraperitoneally (1 mg/kg) every two days to induce chronic endotoxemia for 4 weeks, followed by an injection every two weeks until the 12th week. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Transduction of mouse astroglial cells with bONE-GO sensor and BRET measurements

… Kinetic traces are represented as change in BRET after subtraction of the baseline signal measured for 30 s before GPCR stimulation [ΔBRET·103 (baseline)]. Where indicated in the figures or figure legends, cells expressing Gαi bONE-GO WT were treated overnight with 0.1 μg/ml pertussis toxin (List Biological Labs, cat#179A). For the concentration-response curve presented in Fig. 7C, the average [ΔBRET·103 (baseline)] of the “Buffer” condition was first subtracted from all traces, and then the area-under-curve (AUC) was calculated in Prism (GraphPad) for each trace, followed by curve fit to a 3-parameter sigmoidal equation.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunohistochemistry

Mice were deeply anesthetized with a mixture of isoflurane and oxygen and received an i.p. lethal dose of exagon (300 mg/kg) and lidocaine (30 mg/kg). Mice were perfused transcardially with phosphate-buffered saline (PBS 1X) and incubated (48 h/4 °C) in 4% paraformaldehyde. Coronal slices were cut at 50 μm and washed three times in PBS 1X before incubation in the blocking solution containing 0.03% Triton X-100 and 10% donkey serum or goat serum. … or with a goat anti-CTb antibody (1/10000, list labs #703), or a rabbit anti-GFP primary antibody (1/1000; Millipore, AB3080), a mouse anti-GAD67 primary antibody (1/500; Millipore MAB5406), a guinea pig anti-PV primary antibody (1/1000, synaptic system, cat#195004). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)