Bacterial Toxin Research Citations

… Diphtheria toxin (DT) List Biological Laboratories Cat#150 …

In vivo Cell Depletion

To ablate specific cells, BDCA2-DTRTg/+, BDCA2-DTRTg/+ Stat1−/−, and non-transgenic littermate controls were injected i.p. with 10 ng/g body weight DT on Day −1, Day 0, and Day +2 pi (Swiecki et al., 2010), unless noted otherwise.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

B. pertussis-specific IgG ELISAs

Levels of pertussis-specific serum IgG were measured as described previously (54). Nunc 96-well plates were coated with 0.2 µg/mL pertussis toxin (PT) (List Biologicals) or HKBp at OD600 0.9. Each plate included a standard curve using WHO international pertussis antiserum (National Institute for Biological Standards and Control), and IgG results were expressed as international units (IU)/mL or relative units (RU)/mL by comparing the results with the linear portion of the standard curve.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active experimental autoimmune encephalomyelitis (aEAE) induction and scoring

aEAE was induced by injecting an emulsion of myelin oligodendrocyte glycoprotein peptide35–55 (MOG35-55 peptide, Genscript), 200 µg per animal, in complete Freund’s adjuvant (CFA, prepared from Incomplete Freund’s Adjuvant, Santa Cruz Biotechnology, USA; supplemented with Mycobacterium Tuberculosis, Difco). In brief, 100 µl emulsion of MOG35 − 55 and CFA was injected subcutaneously in mouse flanks (80 µl) and at the tail base (20 µl) at day 0 under short isoflurane anesthesia. A second group of mice were injected subcutaneously with 100 µl emulsion of MOG35 − 55 and CFA into the shoulder area (50 µl on each side). Moreover, 400 ng PTX (List Biological Laboratories, Campbell, CA, USA) was applied intraperitoneally at day 0 and day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Two weeks later, an anterograde axon tracer, cholera toxin B subunit (List Biological Laboratories, Cat # 104; Campbell, CA, USA), was injected into the vitreous cavity 3 days before euthanizing to label optic nerve fibers. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

CCR2+ inflammatory monocyte and CD11c+ cell depletion

The CCR2 depleter (CCR2-DTR) and CCR2 reporter (CCR2-GFP) strains were generated on the C57BL/6 (WTB6) background as previously described (48). Control animals for CCR2-DTR experiments were sex and age-matched, nontransgenic littermates. CD11c-DTR mice in C57BL/6 background were from Dr. Kumamoto’s Laboratory at Rutgers University. CD11c-DTR mice were generated with a targeting construct containing a floxed DTR-IRES-GFP cassette containing a splice acceptor that was knocked into the first intron of the CD11c gene, known as integrin (Itgax) (62). For depletion of CCR2+ or CD11c+ cells, CCR2-DTR mice and control CCR2-DTR negative littermates; or CD11c-DTR mice and their littermate controls, received 250 ng of diphtheria toxin i.p. 1 day prior to infection and every other day thereafter in order to maintain depletion. Diphtheria Toxin was purchased from List Biological Laboratories (Campbell, CA), and reconstituted at 1 mg/mL in PBS.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Immunizations

C57Bl/6 mice (n = 6 per group) were immunized three times, three weeks apart with the following: group 1) DTaP (1/5th human dose of Infanrix, GlaxoSmithKline Biologicals, Rixensart, Belgium); group 2) aP acellular pertussis (aP) biosimilar composed of 5 μg of pertussis toxin mutant (Creative Diagnostics, Shirley, NY), 1.6 μg of pertactin (List Labs, Campbell, CA), …

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

… During the first immunization, mice were also administered 500 ng of pertussis toxin (List Biological Laboratories, Cat#180) through an IP injection. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer