Bacterial Toxin Research Citations

Reagents:

For the bilayer lipid measurements, PA 63 was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA).

Channel Reconstitution into Planar Lipid Bilayers:

…Single channels were formed by adding 0.5 to 1 L of 20g mL 1 solution of PA63, or 0.2 to 0.5 L of 48 ng mL 1 solution of C2IIa to the 1.5 mL aqueous phase on the cis-half of the bilayer chamber. For multichannel experiments, we applied 12 L of 0.2 mg mL1 stock PA63 or 12 L of 48g mL 1 stock C2IIa to the cis-side of the membrane. Under this protocol, PA63 and C2IIa channel insertions were always
directional as judged by channel conductance asymmetry in the applied transmembrane voltage…In most of the experiments, the PAMAM dendrimers were added to the cis-compartment of a bilayer chamber, which was the side of PA63 and C2IIa addition.

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

 Immunization protocols and collection of serum samples:

Cholera antigens (Table 2) were … CT (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… 4. Pertussis toxin (PT) from Bordetella pertussis, lyophilized in buffer (List Biological Laboratories, Inc.). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 7. Pertussis Toxin, if required (see Table 1; List Biologicals). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Material und Methodik … Ultra Pure LPS (Salmonella minessota R595) List Biological Laboratories, Campbell, USA …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Lung inflammation:

LPS (Escherichia coli 055:B5; List Biological Laboratories, Campbell, CA) in a dose of 20 g in 50 l of PBS was instilled using a modified feeding needle into the trachea of mice. Mice were sedated with isoflurane (Baxter, Deerfield, IL) before instillation. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Sensitization model:

Murine sensitization protocol was similar to previously described one (Kanjarawi et al., 2011). BALB/c mice were intragastrically (ig) sensitized with 5 mg of BLG or CL-BLG with 15 µg of Cholera Toxin (CT, List Biological Laboratories, Inc., CA) on days 0, 7, 14, and 21 (n = 8/group). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of oral tolerance and immunization:

In some experiments, the mice were immunized intragastrically with 10mg of OVA and 10µg of cholera toxin (List Biological Laboratories, Campbell, CA).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Animals:

Two male macaque monkeys, one Macaca fascicularis (MF), aged 20, and one Macaca mulatta (MM1), aged 5, were used for the tracer study. Two male Macaca mulatta, one aged 8 (MM2) and one aged 2.5 years (MM3) were used for the detailed retina analyses. The animals were kept in the primate animal facility of the University of Alabama at Birmingham in 12:12 light:dark conditions. For the tracing study, MM1 and MF received a unilateral injection of 100 l of 1% cholera toxin B (CtB) (List Biological) in sterile water into the vitreous body using a 1cc tuberculin syringe under isoflurane anaesthesia. …

Antibody Characterization:

…A commercially available anti-CtB antibody (code no. 703, Lot#7032E, List Biologicals, Champbell, CA, USA, diluted 1:1000) raised in goat was used for tracer visualization. According to the manufacturer the antigen was a B subunit pentamer of cholera toxin (choleragenoid). Omission of the primary antibody abolished all staining (not shown). …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… LPS (E.-coli-derived, serotype O111:B4) was from List Biological Laboratories (Campbell, CA); …

• Product #201 – LPS from Escherichia coli O111:B4