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4165 total record number 127 records this year

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4165 citations found

NLRP3 licenses NLRP11 for inflammasome activation in human macrophages

Gangopadhyay, A;Devi, S;Tenguria, S;Carriere, J;Nguyen, H;Jäger, E;Khatri, H;Chu, LH;Ratsimandresy, RA;Dorfleutner, A;Stehlik, C;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • Cell culture

    … Where indicated, cells were also treated with nigericin (5 μM, Invivogen, 10–45 min), CTB (20 μg ml−1, List Biological Laboratories, 6 h), silica (200 μg ml−1, Invivogen, 6 h), TcdB (10 μg ml−1, R&D Systems, 8 h) and ATP (5 mM, Millipore-Sigma, 25 min); cultured in K+-free medium (0.8 mM MgCl2, 1.5 mM CaCl2, 10 mM HEPES, 5 mM glucose and 140 mM NaCl, pH 7.2, 3 h)50; or transfected with flagellin (500 ng ml−1, Invivogen, 4 h), poly(dA:dT) (1 μg ml−1, Invivogen, 4 h) or FSL-1 (0.2 μg ml−1, Invivogen, 4 h) and ultrapure LPS (1 μg ml−1, 4 h) with Lipofectamine 2000 (Invitrogen) or as otherwise indicated.

    Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

A vaccine targeting resistant tumours by dual T cell plus NK cell attack

Badrinath, S;Dellacherie, MO;Li, A;Zheng, S;Zhang, X;Sobral, M;Pyrdol, JW;Smith, KL;Lu, Y;Haag, S;Ijaz, H;Connor-Stroud, F;Kaisho, T;Dranoff, G;Yuan, GC;Mooney, DJ;Wucherpfennig, KW;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Interspecies commensal interactions have nonlinear impacts on host immunity

Rice, TA;Bielecka, AA;Nguyen, MT;Rosen, CE;Song, D;Sonnert, ND;Yang, Y;Cao, Y;Khetrapal, V;Catanzaro, JR;Martin, AL;Rashed, SA;Leopold, SR;Hao, L;Yu, X;van Dijk, D;Ring, AM;Flavell, RA;de Zoete, MR;Palm, NW;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

AGBE: a dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns

Liang, Y;Xie, J;Zhang, Q;Wang, X;Gou, S;Lin, L;Chen, T;Ge, W;Zhuang, Z;Lian, M;Chen, F;Li, N;Ouyang, Z;Lai, C;Liu, X;Li, L;Ye, Y;Wu, H;Wang, K;Lai, L;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

  • Diphtheria toxin (DT) and puromycin treatments in vitro

    Transfected HEK293 cells for human diphtheria toxin receptor (hDTR) mutagenesis were selected with 20 ng/ml DT (List Labs, #150) from day 3 after electro-transfections. Cell clones mutated in hDTR-sgRNA-1 or hDTR-sgRNA-2 targeted regions were cultured with DT-supplemented growth medium from day 1 after cell sorting (day 0 refers to the day of cell sorting). Transfected PFFs were selected with 300 ng/ml puromycin-containing (MPBIO, 219453925) medium for 48 h after electro-transfections of 12 h. Both DT and puromycin-supplemented growth medium were exchanged daily until negative control cells died completely.

    Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

STAT1 signaling protects self-reactive T cells from control by innate cells during neuroinflammation

Arbelaez, CA;Palle, P;Charaix, J;Bettelli, E;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Food Allergy-Induced Autism-Like Behavior is Associated with Gut Microbiota and Brain mTOR Signaling

Cao, LH;He, HJ;Zhao, YY;Wang, ZZ;Jia, XY;Srivastava, K;Miao, MS;Li, XM;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • Whey Sensitization/Challenge Protocol and Assessment of Anaphylaxis

    The model of cow’s mike allergy-induced autism was established as described by de Theije’s et al, with modifications.37 Whey is a milk protein fraction composed of several milk allergens (β-lactoglobulin, α-lactalbumin, bovine lactoferrin, bovine serum albumin, and bovine immunoglobulins), some of which are major allergens, and used to induce our food allergy model.38 After 1 week acclimation, mice were sensitized intragastrically (i.g) with 20 mg whey (Hefei Bomei Biotechnology Co., LTD, China) in 0.5 mL of PBS plus 10 μg cholera toxin (CT, List Biological Laboratories, Campbell, CA, USA) as an adjuvant from week 1 through week 5 and oral 100 mg whey containing 10 μg CT from week 6 through week 7. Mice were then sensitized via intraperitoneal injection (i.p.) with 500 μg whey in 0.2 mL of PBS containing 2 mg Alum (ThermoFisher, USA) from week 8 through week 9. Naive mice received PBS alone. Sham mice received CT and alum alone. A schematic for the sensitization and challenge timeline was depicted in Figure 1. …

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Polarized macrophages promote gestational beta cell growth through extracellular signal-regulated kinase 5 signalling

Jiang, Y;Chen, A;Kline, D;Liu, Q;Ma, J;Wang, Y;Zhang, T;Qian, J;Nelson, L;Prasadan, K;Hu, B;Gittes, GK;Xiao, X;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Identification of two lipid phosphatases that regulate sphingosine-1-phosphate cellular uptake and recycling

Kono, M;Hoachlander-Hobby, LE;Majumder, S;Schwartz, R;Byrnes, C;Zhu, H;Proia, RL;

Product: Shiga Toxin 2 from Escherichia coli

Enhancement of Regnase-1 expression with stem loop-targeting antisense oligonucleotides alleviates inflammatory diseases

Tse, KM;Vandenbon, A;Cui, X;Mino, T;Uehata, T;Yasuda, K;Sato, A;Tsujimura, T;Hia, F;Yoshinaga, M;Kinoshita, M;Okuno, T;Takeuchi, O;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer