Citations

EAE:

…0.2 ml inoculum containing 200 µg MOG peptide 3555 (MEVGWYRSPFSRVVHLYRNGK) in IFA (DifcoLabs, Detroit, MI) with 800 µg Mycobacterium tuberculosus hominis H37Ra (MT) on day 0 and 7 following [36], with pertussis toxin (PTx) (List Biologicals) 200 ng ip on …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…Mice received ip injections of 500 ng pertussis toxin (List Biological Laboratories, CA) on days 0 and 2. Normal mice were anesthetized and received sterile PBS injections of equal volume in place of MOG 3555 and pertussis immunizations. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

Pertussis toxin was purchased from List Biological Laboratories.

EAE induction and adoptive transfer:

Mice were immunized s.c. with 150 µg MOG₃₅₅₅ in IFA containing 6 mg/ml heat-killed H37RA strain of M. tuberculosis. On days 0 and 2 after immunization, mice received 200 and 400 ng PTX i.p., respectively. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The right panels are the coronal sections of the rat brain showing the afferent projection to the medial amygdaloid nucleus. CTb (1% solution in distilled water; List Biological Laboratories Inc. Campbell, CA …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Following topical application of tetracaine hydrochloride to the cornea, they received a unilateral intraocular injection of CTb (List Biological Laboratories, Inc., Campbell, CA, USA). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Standard stereotaxic surgery and a flat skull approach were used to make a single microinjection of the retrograde tracer CTB (1% in 200 nl saline; List Biological, Campbell, CA) unilaterally into the RVLM as previously described (Oshima et al.,2006). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Iontophoretic tracer delivery:

Rats were anesthetized by halothane or isoflurane inhalation… A solution of 1% cholera toxin subunit B (CTB; List Biological Labs, Campbell, CA, USA) in 0.1 M phosphate buffer (pH 6.0) or… During the descent of the glass pipette into the brain, a 0.5 A retaining current was used to minimize molecular diffusion of tracer from the pipette tip. CTB was unilaterally iontophoresed into the BSTvl (from bregma: 0.3 posterior, +2.8 lateral, 7.0 ventral; 10 angle) or…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Dieser Effekt konnte unabhngig vom Dt-Hersteller (Merck, List Biological Laboratories, Sigma-Aldrich) und in unterschiedlich hohen Dosen (1 g, 0,5 g sowie 0,1 g pro Maus) demonstriert werden. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Pertussis-Toxin; List Biological Laboratories, Campbell, CA, USA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

LCA Endopeptidase Substrate Calibration:

A calibration curve for the SNAPtide used in the LCA endopeptidase kinetics was created using Product #528 (List Biological Labs), an unquenched SNAPtide peptide. The following concentrations of unquenched SNAPtide were prepared in 100.0 L total volume using 20 mM HEPES, 0.1% v/v TWEEN-20, pH 7.6 (assay buffer): 0.0125, 0.0250, 0.0500, 0.100, 0.200, 0.400, 0.600 and 0.800 M. …

Fluorescence Internal Quenching (FIQ) Correction Factor:

A SNAPtide stock solution (2.5 mM in DMSO, List Biological Labs, Product #521) was diluted to 0.200 mM in the assay buffer before further dilutions. The unquenched SNAPtide stock solution (500 M in DMSO) was diluted to 50.0 M in assay buffer prior to further dilutions. …

• Product #528 – Unquenched Calibration Peptide for SNAPtide 521 Substrate for C. botulinum Type A Neurotoxin
• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin