Citations

 Formulation of adjuvants:

Cholera toxin (List Biological Labs) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… In the 12 hours after the chloral hydrate (500mg/kg) was subjected to perfusion fixation under general anesthesia, to prepare frozen sections of the brain and spinal cord. Blocking (0.3% hydrogen peroxide/PBS for 30 minutes, in addition, 3% NDS + 0.25% TX/PBS at room temperature, 1 hour), goat anti-CTB antibody (List biologicals) at room temperature …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Cholera toxin B, Cholera toxin B subunit (cholerogenoid), Goat serum, List Biological Laboratories, Campbell, CA; …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Immunohistochemistry:

… For visualization of Fos in CTb neurons, Fos was stained as described above followed by incubating the same tissue sections in goat anti-CTb (1:20,000 List Labs) overnight, and then biotinylated donkey anti-goat secondary (1:500, Jackson), followed by ABC (1:500). Finally, sections were incubated in DAB to yield a brown reaction product in the cytoplasm.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Lipopolysaccharide (LPS) from Salmonella minnesota R595 was purchased from List Biological Laboratories (Cambell, CA). …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Reagents:

Escherichia coli K12 D31m4 LPS was obtained from List Biological Laboratories …

Human intestinal biopsies:

… Biopsies were subjected to hypoxia as described. After 2h, samples were washed 3 times with prewarmed 0.9% NaCl, incubated for 2h in cell culture medium in the absence or presence of 100ng/ml LPS and analysed. Immunostaining for HIF1 and IRAK1 was performed on tissue sections from patients undergoing tissue resection following mesenteric infarction or cancer.

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

… RASFs and primary peripheral monocytes from healthy individuals were plated in 12- and 24-well plates (5 x 104 and 3-5 x 105 cells/well) in 500 l supplemented DMEM and RPMI, respectively, and stimulated for the indicated time points with the following agents: 300 ng/ml Pam3CSK4, 10 g/ml poly(I:C) (both InvivoGen, San Diego, CA), 10 ng/ml lipopolysaccharide (LPS from Escherichia coli J5; List Biologicals, Campbell, CA), …

• Product #301 – LPS from Escherichia coli  J5 (Rc)

… LPS from Salmonella typhimurium was purchased from List Biological Laboratories, Inc. (Campbell, Ccalifornia). …

• Product #225 – LPS from Salmonella typhimurium

… LPS was purchased from List Biological Lab (San Jose, CA, USA) …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Each conditioned medium was sterile ltered and 10% v/v was added to the control media for subsequent cell treatment. For positive cell activation controls, 1 mg/ml LPS (endotoxin, List Biological Laboratories Inc., Campbell, USA) was added to control media. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/