Bacterial Toxin Research Citations

Epicutaneous sensitization and oral immunization:

… 6 Briefly, 4- to 6-week-old mice were enterally (subsequently referred to as orally) immunized by means of gavage once a week for 7 weeks with 5 mg of OVA and 10 µg of cholera toxin (CT; azide free; List Biological Laboratories, Campbell, Calif) in 150 µL of normal saline or …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents:

LPS (List Biological Laboratories, Campbell, CA) was resuspended in water and sonicated for three 10-sec pulses at 100% duty cycle output 6 …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE Induction:

C57BL/6N mice (sex: male; age: 13 weeks) were immunized subcutaneously with 50 µg myelin oligodendrocyte glycoprotein (MOG) peptide 3555 (Sigma-Genosys; St. Louis, MO) in 50 µl H 2 O emulsified in 50 l CFA (Sigma-Aldrich; St. Louis, MO) that was enriched with 10 µg/ml Mycobacterium tuberculosis (H37Ra; Difco, Detroit, MI) at day 0 to induce EAE. In addition, 200 ng pertussis toxin (List Biological Laboratories; Campbell, CA) was administered intraperitoneally at days 0 and 2. EAE paralysis was scored as follows: 0, no disease; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Each mouse also received an intraperitoneal injection of Pertussis toxin (PTX) (List Biological Labs, Campbell, CA) (150 ng) in 0.9% sterile buffered saline on day 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE in DR2-Tg and MBP-TCR/DR2-Tg mice:

…In addition, mice were given pertussis toxin (Ptx) from List Biological Laboratories (Campbell, CA) on days 0 and 2 post-immunization (75 ng and 200 ng per mouse, respectively).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of Cholangitis.

…injected intraperitoneally (IP) into 6 week-old female B6 mice. Additionally, mice received 100 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) in 100 µL of PBS (IP) at the time of, and 2 days after, the initial immunization with 2OA-BSA.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Animals were immunized s.c. in the abdominal flank with 200 µl myelin oligodendrocyte glycoprotein peptide (MOG3555) (UCLA Biopolymers Core) emulsified in complete Freunds adjuvant (Sigma-Aldrich), followed by i.p. injection of 200 µl pertussis toxin (List Biological Laboratories) on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

B6 mice were immunized s.c. (into the flanks) with 100 µg MOG35-55p…Animals received 200 ng pertussis toxin i.v. (List Biological Laboratories) on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Depletion of TAMs in Braf-induced thyroid cancer mouse models:

Macrophage depletion in the bone marrow (BM), blood, spleen and peritoneal cavity was examined in Ccr2-DTR mice after treatment with diphtheria toxin (DT) 20 ng/g (List Biologicals, Campbell, CA ) intraperitoneally (i.p.) on alternating days for 7 days. Twenty-four hours following the last dose of DT, mice were euthanized by CO2 inhalation and tissue samples obtained for flow cytometry and/or immunohistochemistry (IHC) as described below.

To assess the effects of TAMs on PTC development, Tg-rtTA/tetO-Braf/Ccr2-DTR mice were fed dox-impregnated chow (2500 ppm; Harlan-Teklad) for 7 days with or without DT i.p. on alternating days beginning on day 0. On day 7 (24 hr after the last dose of DT), mice were euthanized by CO₂ inhalation and thyroids extracted for IHC. To examine the role of TAMs in established BRAF-induced thyroid cancers we treated Tg-Braf/Ccr2-DTR mice at approximately 6 and 12 weeks of age with or without DT on alternating days for 10 days. Mice were euthanized and thyroids extracted 24 hr after the last dose of DT for flow cytometry and IHC.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials:

…Staphylococcal enterotoxin B (SEB) was purchased at List Biological Laboratories (Campbell, CA),…

LC-MS:

… To avoid undesirable oxidation of cysteines by air oxygenation, digested SEB samples were further treated with 10 mM TCEP [tris(2-carboxyethyl)phosphine] and then with 100 mM iodoacetamide…