Bacterial Toxin Research Citations

TLR stimulation and ELISA:

Macrophages or DCs were plated onto 96-well tissue culture-treated plates at 5 10⁴ cells/well and allowed to rest for 16 h prior to stimulation. …TLR agonists used were as follows: S. Minnesota R595 Ultra Pure LPS (List Biological Laboratories), …

Flow cytometry and intracellular cytokine staining:

Macrophages were stimulated with LPS for up to 8 h in the presence of 10 g/mL Brefeldin A for the final 2 h of stimulation. …

LPS injection:

Mice were injected with 1 g/g LPS i.p. in PBS and blood was collected at indicated time points. Serum concentrations of IL-12 p40, IL-6, TNF, and IL-10 were determined by ELISA.

Gene expression analysis:

Total RNA from LPS or TNF (Peprotech) stimulated macrophages was isolated using the RNeasy Plus kit (Qiagen) and reverse transcribed with…

ChIP:

ChIP was conducted as described in [35] with minor variations. Briefly, macrophages were stimulated with 1 ng/mL LPS for 8 h, washed and fixed with a 1% final concentration of formaldehyde…

• Product #434 – ULTRA PURE LPS from Salmonella minnesotaR595 (Re)

Materials:

Pertussis toxin (PTx) was from List Biological Laboratories (Campbell, CA).

Results:

…To determine whether the prolonged B2R-mediated NO response in cytokine-treated cells was dependent on coupling through G_i/o, we measured NO production from control and cytokine-treated HLMVEC pretreated with 1.5 µg/ml PTx for 3 h prior to stimulation with BK. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… pertussis toxin (List Biological laboratories, Campbell …

Macrophage Culture and Phagocytosis of A:

PBMCs were isolated by Ficoll-Hypaque gradient technique from venous blood and were differentiated into macrophages (714 days) in IMDM medium (Thermo) with 10% autologous serum as described [11] [12]. Replicate AD macrophage cultures were pre-treated with either U0126 (72 nM), Wortmannin (500 nM), PKI (500 nM), PTX (1 µg/ml), EGTA, MK (10⁷M), anti-GPR32 (Chr32, 1/200), anti-PDIA3 (1/400), or …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice and EAE induction:

…At 8-16 weeks of age mice received a subcutaneous injection of 125 µg MOG₃₅₅₅ peptide (Mimotopes, Australia) emulsified 1:1 (vol/vol) in Complete Freunds Adjuvant containing 4mg/ml Mycobacterium tuberculosis H37Ra (Difco, Detroit, MI), to both flanks and the base of the tail. Pertussis toxin (300ng in PBS; List Biological, USA) was injected intraperitoneally at the time of induction and a second dose was administered three days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… co-cultured overnight with CFSE-JAWS II cells. As a positive control, JAWS II cells were treated overnight with 10 g/mL LPS (Salmonella minnesota from List Biological Laboratories, Inc.). …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

…Briefly, confluent J774A.1 cells of 7 10 4 cells/well were plated in sterile clear bottom black 96-well plates (Costar; Corning, Tewksbury, MA) at 37C in 5% CO 2 . A solution containing 100 ng/mL LF (List Biological Laboratories, Campbell, CA …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

… Pups in Group 4 received a single dose of the HB-EGF antagonist CRM197 (5 mg/kg; List Biological Laboratories, Inc., Campbell, CA, USA) injected intraperitoneally immediately after birth. Survival of all pups was recorded daily. …

• Product #149A – Diphtheria Toxin CRM₁₉₇, Mutant

… Mice (n=6) were immunized sl with 20 µg of sHA1 protein alone, or with 2 µg of cholera toxin (CT) (LIST BIOLOGICAL LABS INC., USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Tract tracing:

For neurosurgery, geckos were maintained at about 25 °C using a heating blanket with feedback control. …

The retrograde tracer neurobiotin (NB, Molecular Probes, WA) diluted 1:200 and the anterograde tracer cholera toxin (CTX, List Biological Laboratories, CA) diluted 1:500 were injected into the VIIIth nerve with tracer-filled glass pipettes. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae