Citations

4.2. Mice and EAE induction

EAE was induced 4 weeks following vector administration in female C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME, USA). Briefly MOG35-55 (200ug) emulsified in Complete Freund’s Adjuvant (Sigma Aldrich, St. Louis, MO) with 9 mg/mL Mycobacterium tuberculosis (Difco Laboratories, Franklin Lakes, NJ) was injected subcutaneously over two sites at the upper and lower back. 2- and 24- hours following immunization, 200 ng Pertussis Toxin (List Biological Laboratories, Inc, Campbell, CA) was injected interperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… In TeNT treatment, an 8-nl mixture containing 0.2M KCL, 0.5% dextran Antonia Red 150,000 MW (Sigma) and TeNT (List Labs, 190A; 0.5M) was pressure injected into the hindbrain ventricle. Between 50 and 70 min after injection, dextran was imaged, followed by another 10 min of GCaMP6s imaging. …

• Product #190 – Tetanus Toxin from Clostridium tetani

… Three days before euthanasia, rats were anesthetized with isoflurane (5% for induction; 2-2.5% for maintenance), and 15-20 µL cholera toxin B subunit (CTB, #104 List Biological Labs, Campbell, California, USA) was bilaterally injected into the intrapleural space to retrogradely label PhMNs, as previously described (Cheng et al 2021a) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis toxin (List Biological Laboratories) was administered intraperitoneally on days 0 and 1 postimmunization. Following an established scoring system, ocular disease progression was assessed using fundus examinations. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Enzyme-linked immunosorbent assay

Whole toxin A (List Biologicals, Campbell, CA) or recombinant rbdB was used to coat U-bottom high-binding 96-well ELISA plates (Greiner Bio-One, Frickenhausen, Germany). …

• Product #152C – Toxin A from Clostridium difficile

… The emulsion was injected subcutaneously into both hind limbs of each animal. The mice were treated intraperitoneally with 200 ng of pertussis toxin from Bordetella pertussis (dissolved in PBS; List Biological Lab, USA) at the time of immunization and 48 hours after …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… All animals received 2 subcutaneous injections of 100 µL each of MOG and a single intraperitoneal injection of 400 ng pertussis toxin (List Biological Laboratories, #180) in 200 µL of PBS. Mice received a second injection of PTx 48 hours after the initial injection. Mice were monitored and clinical scores were …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction of EAE

Active EAE was induced following previously published procedures [23]. In brief, 8–10-week-old female C57BL/6J mice (#000664, The Jackson Laboratory) were injected subcutaneously in both flanks with a total of 100 μg of MOG35-55 peptide (EZBiolab) in complete Freund’s adjuvant (CFA) containing 4 mg/mL Mycobacterium tuberculosis (DIFCO Laboratories). Mice also received 400 ng of pertussis toxin (LIST Biological Laboratories) intraperitoneally both immediately after immunization and 48 h later. Control, mock-injected mice were injected with everything except the MOG peptide. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Pertussis toxin List Biological Labs Cat #179 A …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of experimental autoimmune orchitis (EAO)

Age-matched male Gsdmdfl/flCx3cr1-cre and Cx3cr1-cre mice were used to induce EAO, as described previously (Nicolas et al, 2017). First, the testicular homogenate (TH) was obtained from decapsulated testes of adult syngeneic mice and homogenized in an equal volume of sterile PBS. Second, TH was mixed with incomplete Freund’s adjuvant (IFA; Sigma-Aldrich, F5506) containing 1 mg/ml Mycobacterium tuberculosis (BD, 231141) in 1:1 ratio. Finally, the mice were immunized thrice every 14 days with the mixture, followed by an intraperitoneal injection of 100 ng pertussis toxin (List Biological Laboratories, lot#180) in 100 μl PBS. Each animal was immunized dorsally at four sites near the popliteal lymph nodes in a total volume of 200 μl. The testes were collected for further analysis 50 days after the first immunization.

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)